Virulence of Phospholipase C Mutants of Pseudomonas aeruginosa PAO1 against the Silkworm, Bombyx mori

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著者

    • Iiyama Kazuhiro IIYAMA Kazuhiro
    • Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture, Graduate School, Kyushu University
    • Chieda Yuuka CHIEDA Yuuka
    • Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture, Graduate School, Kyushu University
    • KUSAKABE Takahiro
    • Laboratory of Silkworm Science, Faculty of Agriculture, Graduate School, Kyushu University
    • YASUNAGA AOKI Chisa
    • Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture, Graduate School, Kyushu University
    • SHIMIZU Susumu
    • Laboratory of Insect Pathology and Microbial Control, Institute of Biological Control, Faculty of Agriculture, Graduate School, Kyushu University

抄録

To estimate the contribution of phospholipase C (PLC) in the virulence of Pseudomonas aeruginosa against <i>Bombyx mori</i>, PlcB, PlcH, and PlcN mutants were created by the sacB-based counterselection method. Insertion of Ωaac cassette (selection marker, Gm<sup>R</sup>) in plcB, plcH, and plcN was confirmed by Southern hybridization and the mutants were designated as PAO1plcB, PAO1plcH, and PAO1plcN, respectively. Inactivation of PLC genes did not cause growth deficient in vitro. The culture filtrates of PAO1, PAO1plcB, and PAO1plcN had hemolytic activity, but not PAO1plcH. When the PLC mutants were inoculated into the fourth instar of <i>Bombyx mori</i> larvae, all mutants maintained high virulence, indicating that at least inactivation of a single plc gene in P. aeruginosa did not affect the virulence against B. mori. It was different from the results obtained in another Lepidopteran host model, Galleria mellonella.<br>

収録刊行物

  • Journal of insect biotechnology and sericology  

    Journal of insect biotechnology and sericology 77(2), 115-120, 2008-06-30 

    The Japanese Society of Sericultural Science

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各種コード

  • NII論文ID(NAID)
    10021221206
  • NII書誌ID(NCID)
    AA11558849
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13468073
  • NDL 記事登録ID
    9574043
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産)
  • NDL 請求記号
    Z54-J696
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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