Somatic Embryogenesis from Cell Suspension Cultures of Taranoki (Aralia elata)
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The objective of this study was to increase the formation rate of embryos from suspension cultures of Taranoki (<i>Aralia elata</i>). The suspension cultures were prepared from leaf-derived calli. Of the various culture durations, 30 days of culture yielded the best growth of cell suspension cultures in a BT liquid medium supplemented with 2,4-D. Somatic embryogenesis has never been observed without the addition of exogenous plant growth regulators (PGRs). The highest percentage of somatic embryo formation (93.3%) was observed in a BT liquid medium supplemented with 1.0 μM BAP+5.0 μM 2,4-D. The PGR-free semi-solid BT medium showed the highest regeneration frequency of cotyledonary-shaped embryos (100%).
- Environment Control in Biology
Environment Control in Biology 45(3), 173-178, 2007-09-30
Japanese Society of Agricultural, Biological and Environmental Engineers and Scientists