A Simple Extraction Method of Soluble Carbohydrates from Various Organs of Several Ornamental Plants Using a Vacuum Manifold

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A simple method using a vacuum manifold for extracting soluble carbohydrates for analysis by high performance liquid chromatography (HPLC) from various tissues of ornamental plants was developed. Rose 'Sonia' petals were immersed in ethanol solution at 75°C for 20 min. Sorbitol was added to the extraction as an internal standard, and extractions were transferred to filter holders on a vacuum manifold, and the manifold was then decompressed. The filtrate was evaporated to dryness, and used for HPLC analysis. In comparison to soluble carbohydrate extraction by conventional homogenization extraction, few differences in soluble carbohydrate content were observed. Carbohydrate content in the remaining plant tissue was confirmed to be very low, indicating that soluble carbohydrates are effectively extracted by this procedure. Few differences in soluble carbohydrate content were observed among rose stem and leaves between the new method and a conventional method. Also, few differences in soluble carbohydrate content were observed among petals or sepals, stems and leaves in carnation, <I>Delphinium</I> and snapdragon, using either the new or conventional method. These findings suggest that the new method, which does not require homogenization, is a very simple method for the extraction of soluble carbohydrates from various organs of several ornamental plants.

収録刊行物

  • Environment control in biology  

    Environment control in biology 46(1), 49-55, 2008-03-30 

    Japanese Society of Agricultural, Biological and Environmental Engineers and Scientists

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各種コード

  • NII論文ID(NAID)
    10021229477
  • NII書誌ID(NCID)
    AA12029220
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    1880554X
  • NDL 記事登録ID
    9478992
  • NDL 雑誌分類
    ZR1(科学技術--生物学)
  • NDL 請求記号
    Z78-A361
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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