Plasma UGRP1 Levels Associate with Promoter G-112A Polymorphism and the Severity of Asthma

• Inoue Keiichi

  Department of Pulmonary Medicine, School of Medicine, Fukushima Medical University

• Wang Xintao

  Department of Pulmonary Medicine, School of Medicine, Fukushima Medical University

• Saito Junpei

  Department of Pulmonary Medicine, School of Medicine, Fukushima Medical University

• Tanino Yoshinori

  Department of Pulmonary Medicine, School of Medicine, Fukushima Medical University

• Ishida Takashi

  Department of Pulmonary Medicine, School of Medicine, Fukushima Medical University

• Iwaki Daisuke

  Department of Immunology, School of Medicine, Fukushima Medical University

• Fujita Teizo

  Department of Immunology, School of Medicine, Fukushima Medical University

• Kimura Shioko

  Laboratory of Metabolism, National Cancer Institute, National Institute of Health

• Munakata Mitsuru

  Department of Pulmonary Medicine, School of Medicine, Fukushima Medical University

Abstract

Background: Uteroglobin-related protein 1 (UGRP1) is a secretory protein expressed in the airways and is speculated to have anti-inflammatory activity. In the mouse, its gene expression is down-regulated by interleukin (IL)-5 and -9, and up-regulated by IL-10. However, the precise role of UGRP1 in human inflammatory airway diseases such as asthma has not been clarified. The objectives of this study were to establish an ELISA system to quantify UGRP1 protein, and to examine whether plasma UGRP1 levels are associated with the G-112A polymorphism, asthma susceptibility, and its severity.<br> Methods: 152 asthma patients and 103 normal controls were involved in this study. Mice were immunized with recombinant UGRP1 and hybridoma cell lines were established. A sandwich ELISA system was established by using two monoclonal antibodies recognizing different epitopes. Plasma UGRP1 levels were measured with the ELISA system and the G-112A allele was detected by using real-time PCR.<br> Results: An ELISA system was established that allowed determination of UGRP1 levels within the range of 9.6-1250pg/ml. The mean plasma UGRP1 levels for subjects with -112A allele were significantly lower than those without it (p = 0.025). Although there was no significant difference in the plasma UGRP1 levels between asthma patients and controls (p = 0.13), severe asthma patients without oral corticosteroid had significantly lower plasma UGRP1 levels compared to mild- or moderate- asthma patients and controls (p = 0.004, 0.03 and 0.003, respectively).<br> Conclusions: The ELISA system for quantifing UGRP1 protein was established, and plasma UGRP1 levels were associated with the G-112A UGRP1 gene promoter polymorphism and the severity of asthma.<br>

Journal

• Allergology International

  Allergology International 57 (1), 57-64, 2008

  Japanese Society of Allergology

Keywords

• asthma
• genetic polymorphism
• plasma levels
• severity
• uteroglobin-related protein 1

Details

• CRID: 1390282679610528768
• NII Article ID: 10021246095
• NII Article ID: 130004476955
• NII Book ID: AA11091750
• DOI: 10.2332/allergolint.o-07-493
• ISSN: 14401592
• ISSN: 13238930
• PubMed: 18089940
• Web Site: http://api.elsevier.com/content/article/PII:S132389301530753X?httpAccept=text/xml
• Web Site: http://api.elsevier.com/content/article/PII:S132389301530753X?httpAccept=text/plain
• Web Site: https://search.jamas.or.jp/link/ui/2008307060
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed