The -1535 Promoter Variant of The Visfatin Gene Is Associated with Serum Triglyceride and HDL-cholesterol Levels in Japanese Subjects

• TOKUNAGA Ayumi

  Department of Metabolic Medicine, Graduate School of Medicine, Osaka University

• MIURA Atsuko

  Department of Metabolic Medicine, Graduate School of Medicine, Osaka University

• OKAUCHI Yukiyoshi

  Department of Metabolic Medicine, Graduate School of Medicine, Osaka University

• SEGAWA Katsumori

  Department of Metabolic Medicine, Graduate School of Medicine, Osaka University

• FUKUHARA Atsunori

  Department of Metabolic Medicine, Graduate School of Medicine, Osaka University

• OKITA Kohei

  Department of Metabolic Medicine, Graduate School of Medicine, Osaka University

• TAKAHASHI Masahiko

  Department of Metabolic Medicine, Graduate School of Medicine, Osaka University

• FUNAHASHI Tohru

  Department of Metabolic Medicine, Graduate School of Medicine, Osaka University

• MIYAGAWA Jun-ichiro

  Division of Diabetes & Metabolism, Internal Medicine, Hyogo College of Medicine

• SHIMOMURA Iichiro

  Department of Metabolic Medicine, Graduate School of Medicine, Osaka University

• YAMAGATA Kazuya

  Department of Metabolic Medicine, Graduate School of Medicine, Osaka University

抄録

Visfatin is a novel adipocytokine that is expressed by the visceral fat cells. We investigated the role of genetic variation in the visfatin gene in the pathophysiology of type 2 diabetes and clinical variables in Japanese subjects. The 11 exons, and the promoter region of the visfatin gene were screened for single nucleotide polymorphisms (SNPs) by PCR-direct sequencing. We found SNPs in the promoter region (SNP - 1535T>C), exon 2 (SNP + 131C>G, Thr44Arg), and exon 7 (SNP + 903G>A). The allele and genotype frequencies of these SNPs showed no significant differences between 200-448 diabetic and 200-333 control subjects. However, the -1535T/T genotype was associated with lower serum triglyceride levels (T/T vs. T/C + C/C (p = 0.015) and T/T vs. C/C (p = 0.043)) and higher HDL-cholesterol levels (T/T vs. C/C, p = 0.0496) in the nondiabetic subjects. Reporter gene assay of 3T3-L1 adipocytes revealed that the promoter activity of -1535T and -1535C was similar, suggesting that the observed association may reflect linkage disequilibrium between -1535T>C and causative variations of the visfatin gene.<br>

収録刊行物

• Endocrine Journal

  Endocrine Journal 55 (1), 205-212, 2008

  一般社団法人 日本内分泌学会

キーワード

• Visfatin
• Type 2 diabetes
• Genetics
• SNP
• Metabolism

詳細情報

• CRID: 1390282681275711744
• NII論文ID: 10021265064
• NII論文ID: 130004443369
• NII書誌ID: AA10901436
• DOI: 10.1507/endocrj.k07e-039
• ISSN: 13484540
• ISSN: 09188959
• Web Site: http://www.jstage.jst.go.jp/article/endocrj/55/1/55_K07E-039/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2008175216
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可