Herbal Medicine, Hachimi-jio-gan, and Its Component Cinnamomi Cortex Activate the Peroxisome Proliferator-activated Receptor Alpha in Renal Cells

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  • MONDEN Tsuyoshi
    Department of Endocrinology and Metabolism, Dokkyo University School of Medicine
  • HOSOYA Takeshi
    Department of Medicine and Molecular Science, Gunma University Graduate School of Medicine
  • NAKAJIMA Yasuyo
    Department of Medicine and Molecular Science, Gunma University Graduate School of Medicine
  • KISHI Mikiko
    Department of Medicine and Molecular Science, Gunma University Graduate School of Medicine
  • SATOH Teturou
    Department of Medicine and Molecular Science, Gunma University Graduate School of Medicine
  • HASHIMOTO Koshi
    Department of Medicine and Molecular Science, Gunma University Graduate School of Medicine
  • KASAI Kikuo
    Department of Endocrinology and Metabolism, Dokkyo University School of Medicine
  • YAMADA Masanobu
    Department of Medicine and Molecular Science, Gunma University Graduate School of Medicine
  • MORI Masatomo
    Department of Medicine and Molecular Science, Gunma University Graduate School of Medicine

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Abstract

Hachimi-jio-gan is widely used to improve several disorders associated with diabetes, but its mechanism remains poorly understood. In an attempt to clarify the mechanism of Hachimi-jio-gan, we investigated the effects of this herbal medicine and its components in transfection studies of CV1 cells, especially nuclear receptor-mediated actions. One half (0.5) mg/ml of Hachimi-jio-gan activated peroxisome proliferator-activated receptor (PPARα), mediating the activation by 3.1-fold on DR1 response elements; however, it did not affect PPARγ, thyroid hormone receptor, androgen receptor, estrogen receptor or RXR. In addition, this activation was observed in a dose-dependent manner. Next, to determine which components of Hachimi-jio-gan activate PPARα-mediated transcription, 8 of its components (rehmanniae radix, orni fructus, dioscoreae rhizoma, alismatis rhizoma, hoelen, moutan cortex, cinnamomi cortex, aconiti) were tested. Only cinnamomi cortex (1.0 mg/ml) increased PPARα-mediated transcription by 4.1-fold, and this activation was specific for PPAR α, and not for other nuclear receptors. Moreover, this PPARα-related activation by cinnamomi cortex is specifically observed in renal cells. Taken together, these findings indicate that Hachimi-jio-gan and cinnamomi cortex may have a pharmacological effect through the target site for PPARα.<br>

Journal

  • Endocrine Journal

    Endocrine Journal 55 (3), 529-533, 2008

    The Japan Endocrine Society

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