A Simple Method to Induce Differentiation of Murine Bone Marrow Mesenchymal Cells to Insulin-producing Cells Using Conophylline and Betacellulin-delta4

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著者

    • PARK KEE-YONG
    • Institute for Molecular & Cellular Regulation, Gunma University
    • YAMADA SATOKO
    • Institute for Molecular & Cellular Regulation, Gunma University
    • MORI MASATOMO
    • Department of Medicine and Molecular Science, Gunma University Graduate School of Medicine
    • SENO MASAHARU
    • Department of Biotechnology, Okayama University Graduate School of Science and Technology
    • UMEZAWA KAZUO
    • Department of Applied Chemistry, Faculty of Science and Technology, Keio University
    • TAKEI IZUMI
    • Department of Internal Medicine, Tokyo Dental College Ichikawa General Hospital
    • KOJIMA ITARU
    • Institute for Molecular & Cellular Regulation, Gunma University

抄録

The present study was conducted to establish a method to induce differentiation of bone marrow (MB)-derived mesenchymal cells into insulin-producing cells. When mouse BM-derived mesenchymal cells were cultured for 60 days in medium containing 10% fetal calf serum and 25 mM glucose, they expressed insulin. Addition of activin A and betacellulin (BTC) accelerated differentiation, and immunoreactive insulin was detected 14 days after the treatment. Insulin-containing secretory granules were observed in differentiated cells by electron microscopy. Treatment of BM-derived mesenchymal cells with conophylline (CnP) and BTC-delta4 further accelerated differentiation, and mRNA for insulin was detected 5 to 7 days after the treatment. Mesencymal cells treated with CnP and BTC-delta4 responded to a high concentration of glucose and secreted mature insulin. When these cells were transplanted into streptozotocin-treated mice, they markedly reduced the plasma glucose concentration, and the effect continued for at least 4 weeks. These results indicate an efficacy of the combination of CnP and BTC-delta4 in inducing differentiation of BM-derived mesenchymal cells into insulin-producing cells.<br>

収録刊行物

  • Endocrine journal  

    Endocrine journal 55(3), 535-543, 2008-06-01 

    The Japan Endocrine Society

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各種コード

  • NII論文ID(NAID)
    10021266505
  • NII書誌ID(NCID)
    AA10901436
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09188959
  • データ提供元
    CJP書誌  J-STAGE 
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