Inhibitory Effects of Cholesterol Sulfate on Progesterone Production in Human Granulosa-like Tumor Cell Line, KGN

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著者

    • TSUTSUMI RYO
    • Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo
    • HIROI HISAHIKO
    • Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo
    • MOMOEDA MIKIO
    • Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo
    • HOSOKAWA YUMI
    • Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo
    • NAKAZAWA FUMIKO
    • Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo
    • KOIZUMI MINAKO
    • Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo
    • YANO TETSU
    • Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo
    • TSUTSUMI OSAMU
    • Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo
    • TAKETANI YUJI
    • Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo

抄録

Cholesterol sulfate (CS) is a component of cell membranes that plays a role in stabilizing the cell membrane. We previously reported that CS increased in the endometrium during implantation, suggesting that CS plays an important role in reproduction. It has been reported that CS regulates progesterone and pregnenolone production in the placenta, adrenal glands and ovary. The regulatory mechanisms of steroid hormone production by CS, however, are still unknown. In the present study, we investigated the effect of CS on the expression of progesterone production-related genes in KGN cells, derived from human granulosa-like tumor. KGN cells were cultured with CS (10 μM) or cholesterol (10 μM) in the presence of 8-bromo-cAMP (1 mM). Progesterone levels in the culture media were measured by enzyme linked fluorescent assay at 24 h after treatment of CS and cAMP. Total RNAs were extracted for quantitative real time RT-PCR with specific primer of StAR protein, P450scc, HSD3B2, ferredoxin and ferredoxin reductase. Whole cell lysates were extracted for western blot analysis with antibody for StAR protein. Progesterone concentration in the culture medium increased to 38-fold by treatment of cAMP. CS significantly reduced progesterone concentration by 30% compared with those of cAMP treatment (p<0.05), while cholesterol did not change the progesterone concentration. CS treatment down-regulated the expression of StAR mRNA and P450scc mRNA was to 54% and 60%, respectively (p<0.05). Western blot analysis revealed that the amount of StAR protein was also reduced by CS treatment. The expression of HSD3B2 mRNA was up-regulated to 3.4-fold by treatment of cAMP. The expression of ferredoxin and ferredoxin reductase mRNA was not affected by CS treatment. These data implied that CS has an inhibitory effect on progesterone production by regulating the expression of StAR and P450scc gene expression.<br>

収録刊行物

  • Endocrine journal  

    Endocrine journal 55(3), 575-581, 2008-06-01 

    The Japan Endocrine Society

参考文献:  23件

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各種コード

  • NII論文ID(NAID)
    10021266639
  • NII書誌ID(NCID)
    AA10901436
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09188959
  • データ提供元
    CJP書誌  J-STAGE 
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