蛍光染色法を用いたRO水製造工程中に存在する細菌の迅速評価 [in Japanese] Rapid enumeration of bacteria in the production process of RO water in a dialysis facility by fluorescent staining methods [in Japanese]
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透析治療に欠かすことのできないRO水の製造工程における細菌現存量の推移を, 蛍光染色法により計測した. 蛍光染色法として蛍光活性染色法 (DAPI-6CFDA二重染色) およびマイクロコロニー法を用い, 現行の培養法やエンドトキシン活性の測定結果と比較した. その結果, RO水製造工程中の細菌現存量計測は現行の培養法のみでは不十分であり, 蛍光染色法を併用して行うことが有用であることが示唆された. また, 各手法の組み合わせにより総合的に評価をすることで, さらに安全性を高めるための注意部位の存在も明らかとなった.
Bacterial numbers during each of 5 steps in the production process of RO water, which dialysis fluid was made from, were measured using fluorescent staining methods (DAPI and 6CFDA staining, and microcolony methods). And measured values were compared with those obtained by conventional methods (existing culture method and measurement of endotoxin activity). According to the results of the comparison, we confirmed that bacterial number in the production process of RO water was not measured precisely by the existing culture methods and combination with fluorescent staining methods was effective for monitoring, as indicated in related reports. As a result of the measurements of the bacterial number at these 5 stages using various methods, we confirmed that the bacterial number required attention at these points from tap water to RO tank in order to ensure the safety of dialysis fluid.
- Journal of Japanese Society for Dialysis Therapy
Journal of Japanese Society for Dialysis Therapy 40(12), 1051-1056, 2007-12-28
The Japanese Society for Dialysis Therapy