Rapid enumeration of bacteria in the production process of RO water in a dialysis facility by fluorescent staining methods

• Naramura Tomotaka

  Ide Clinic

• Sato Kazuhiro

  Ide Clinic

• Matsuura Hiromi

  Ide Clinic

• Tokura Hideomi

  Ide Clinic

• Inoguchi Aki

  Ide Clinic

• Koga Hisae

  Ide Clinic

• Ide Takao

  Ide Clinic

Bibliographic Information

Other Title

• 蛍光染色法を用いたＲＯ水製造工程中に存在する細菌の迅速評価
• ケイコウ センショクホウ オ モチイタ ROスイ セイゾウ コウテイチュウ ニ ソンザイスル サイキン ノ ジンソク ヒョウカ

Abstract

Bacterial numbers during each of 5 steps in the production process of RO water, which dialysis fluid was made from, were measured using fluorescent staining methods (DAPI and 6CFDA staining, and microcolony methods). And measured values were compared with those obtained by conventional methods (existing culture method and measurement of endotoxin activity). According to the results of the comparison, we confirmed that bacterial number in the production process of RO water was not measured precisely by the existing culture methods and combination with fluorescent staining methods was effective for monitoring, as indicated in related reports. As a result of the measurements of the bacterial number at these 5 stages using various methods, we confirmed that the bacterial number required attention at these points from tap water to RO tank in order to ensure the safety of dialysis fluid.

Journal

• Nihon Toseki Igakkai Zasshi

  Nihon Toseki Igakkai Zasshi 40 (12), 1051-1056, 2007

  The Japanese Society for Dialysis Therapy

Keywords

• RO水
• 細菌
• 蛍光染色
• マイクロコロニー
• 迅速

Details

• CRID: 1390282679654415104
• NII Article ID: 10021274203
• NII Book ID: AN10432053
• DOI: 10.4009/jsdt.40.1051
• ISSN: 1883082X
• ISSN: 13403451
• NDL BIB ID: 9318408
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9318408
• Web Site: https://search.jamas.or.jp/link/ui/2008119197
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed