共鳴効果を利用する生体分子の部位特異的ダイナミクス観測 [in Japanese] Site-specific Observation of Dynamics in Biological Molecules Utilizing Resonance Effect [in Japanese]
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Protein dynamics are intimately connected to the structure/function relationship of biological systems. The dynamics spans over a wide range of time scales from the earliest moments, such as the picosecond regime, toward time scales highly relevant to biological functions, such as the microsecondor millisecond regimes. We have been studying protein dynamics by using time-resolved resonance Raman spectroscopy, which is sufficiently structure sensitive at a chemical-bond resolution. One of the advantages of resonance Raman spectroscopy over other spec-troscopy is selectivity due to resonance effect. By taking advantage of resonance effect, we can investigate specificparts of protein by using different excitation wavelengths. Thus, this technique is suitable for examining how proteins changetheir shape in response to external stimuli. In this review, we summarize recent progresses in resonance Raman studies on protein dynamics.
- Bunko Kenkyu
Bunko Kenkyu 57(4), 179-194, 2008-08-15
The Spectroscopical Society of Japan