RFLP Analysis of Mitochondrial DNA in Fourteen Laboratory Strains of Japanese Quail
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Restriction fragment length polymorphism (RFLP) of mitochondrial DNA (mtDNA) of 14 males and 14 females from 14 laboratory strains of Japanese quail was analysed using 15 restriction endonucleases that recognize six base pairs. Of the 15 restriction endonucleases, only BamH I revealed RFLP in the 14 strains. These 14 strains were divided into two mtDNA haplotypes, A (5 strains) and B (9 strains). The nucleotide sequence divergence estimated between the two haplotypes was 0.00230. BamH I could be a good indicator for elucidating the phylogenetic relationships among various commercial populations or laboratory strains domesticated from wild Japanese quail. The present result on RFLP analysis of mtDNA implies a possibility that the laboratory strains of Japanese quail had been established from at least two different gene pools.
- Nihon Chikusan Gakkaiho
Nihon Chikusan Gakkaiho 71(2), 123-129, 2000-03-25
Japanese Society of Animal Science