A New Screening Method for Probiotic Lactic Acid Bacteria in the Lactobacillus acidophilus Group by Using Carbohydrate Probes

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抄録

To select probiotic strains of the Lactobacillus (L.) acidophilus group with high adhesion to human intestinal tracts for developing the functional yogurt, a new screening method using biotinylated carbohydrate probe (BCP) was introduced. After incubation of living cells of 30 strains of the L. acidophilus group with 5 kinds of BCPs which possess representative partial structures of the sugar chains constituting of human colonic mucin, the interaction between cell-surface lectins and BCP were evaluated. The highest reactivity was detected in 19 strains (about 63%) with a trisaccharide probe (GalNAcα (1→3)[Fucα (1→2)] Galβ 1→). The cell-surface lectin activity was first clearly confirmed in the B group of the L. acidophilus group. The adhesion evaluation score (AE score, total sum of each absorbance of 5 BCPs samples) is considered to be useful as an indicator for selection of the probiotic strains which have high adhesiveness in human gastrointestinal tract.

収録刊行物

  • 日本畜産學會報 = The Japanese journal of zootechnical science  

    日本畜産學會報 = The Japanese journal of zootechnical science 71(1), 103-108, 2000-01-25 

    Japanese Society of Animal Science

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各種コード

  • NII論文ID(NAID)
    10021722226
  • NII書誌ID(NCID)
    AN00195188
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    1346907X
  • データ提供元
    CJP書誌  CJP引用  J-STAGE 
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