A New Screening Method for Probiotic Lactic Acid Bacteria in the Lactobacillus acidophilus Group by Using Carbohydrate Probes
To select probiotic strains of the Lactobacillus (L.) acidophilus group with high adhesion to human intestinal tracts for developing the functional yogurt, a new screening method using biotinylated carbohydrate probe (BCP) was introduced. After incubation of living cells of 30 strains of the L. acidophilus group with 5 kinds of BCPs which possess representative partial structures of the sugar chains constituting of human colonic mucin, the interaction between cell-surface lectins and BCP were evaluated. The highest reactivity was detected in 19 strains (about 63%) with a trisaccharide probe (GalNAcα (1→3)[Fucα (1→2)] Galβ 1→). The cell-surface lectin activity was first clearly confirmed in the B group of the L. acidophilus group. The adhesion evaluation score (AE score, total sum of each absorbance of 5 BCPs samples) is considered to be useful as an indicator for selection of the probiotic strains which have high adhesiveness in human gastrointestinal tract.
- 日本畜産學會報 = The Japanese journal of zootechnical science
日本畜産學會報 = The Japanese journal of zootechnical science 71(1), 103-108, 2000-01-25
Japanese Society of Animal Science