A New Screening Method for Probiotic Lactic Acid Bacteria in the Lactobacillus acidophilus Group by Using Carbohydrate Probes

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  • New Screening Method for Probiotic Lactic Acid Bacteria in the Lactobacillus acidophilus Group by Using Carbohydrate Probes

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Abstract

To select probiotic strains of the Lactobacillus (L.) acidophilus group with high adhesion to human intestinal tracts for developing the functional yogurt, a new screening method using biotinylated carbohydrate probe (BCP) was introduced. After incubation of living cells of 30 strains of the L. acidophilus group with 5 kinds of BCPs which possess representative partial structures of the sugar chains constituting of human colonic mucin, the interaction between cell-surface lectins and BCP were evaluated. The highest reactivity was detected in 19 strains (about 63%) with a trisaccharide probe (GalNAcα (1→3)[Fucα (1→2)] Galβ 1→). The cell-surface lectin activity was first clearly confirmed in the B group of the L. acidophilus group. The adhesion evaluation score (AE score, total sum of each absorbance of 5 BCPs samples) is considered to be useful as an indicator for selection of the probiotic strains which have high adhesiveness in human gastrointestinal tract.

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