書誌事項
- タイトル別名
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- Sex Determination of Equine Somatic and Germ Cells by PCR Amplification Based on the Sequence Polymorphism of X- and Y-Chromosomal Amelogenin Genes
- アメロゲニン イデンシ ノ タケイセイ オ シヒョウ ト シタ ウマ ノ タイサイボウ オヨビ セイショク サイボウ ノ セイベツ ハンテイホウ
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抄録
Two distinct transcripts for equine X- and Y-chromosomal amelogenin genes were cloned from male and female fetal ameloblast cDNA libraries. The complete nucleotide sequence of equine X-chromosomal amelogenin cDNA was 696 -base-pairs (bp) that encodes a protein with 192 amino acid residues. The deletion of 24bp was found in the fifth exon of equine Y-chromosomal amelogenin gene. The genomic DNAs of equine male and female were amplified by the polymerase chain reaction using a pair of nucleotide primers from an X/Y-homologous region of amelogenin gene. The PCR product from mare genomic DNA revealed a single band (184bp) corresponding to the X-chromosome by 4% agar gel electrophoresis. And the PCR products from stallion and gelding genomic DNA showed three bands which were two distinct bands (184bp and 160bp) with X-and Y-chromosome and an additional band (-200bp) of heteroduplex. This PCR polymorphism of X-and Y-chromosomal amelogenin genes enabled sex determination of single sperm as well as equine culture cells.
収録刊行物
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- 日本畜産学会報
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日本畜産学会報 70 (7), 6-10, 1999
公益社団法人 日本畜産学会
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詳細情報 詳細情報について
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- CRID
- 1390282680173286528
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- NII論文ID
- 130000742819
- 10021722776
- 10020392001
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- NII書誌ID
- AN00195188
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- ISSN
- 18808255
- 00215309
- 1346907X
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- NDL書誌ID
- 4670976
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可