Collagen-Carboxymethyl Dextran Conjugate without Platelet Aggregation

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著者

    • HATTORI Makoto
    • Faculty of Agriculture, Tokyo University of Agriculture and Technology
    • TAKAHASHI Koji
    • Faculty of Agriculture, Tokyo University of Agriculture and Technology

抄録

Pepsin solubilized collagen-carboxymethyl dextran (PSC-CMD) conjugate was prepared to improve the function of collagen by using cyanogen bromide. The conjugation between PSC and CMD was confirmed by SDS-PAGE, isoelcctric focusing and size-exclusion chromatography. The molar ratio of the conjugate was PSC:CMD=1:2.3. The isoelectric point was shifted from 9.0 to 5.5 by conjugation. Denaturation temperature of the conjugate molecule was 42.2°C, which is 1.9°C higher than that of PSC. The PSC-CMD conjugate maintained self-assembling ability but showed a very slow aggregation as compared with that of PSC. Reconstructed matrix of the conjugate had lower denaturation temperature and random molecular rearrangement structure as compared with PSC matrix. The platelet aggregation inducing ability, analyzed by spectroscopy disappeared.

収録刊行物

  • 日本畜産學會報 = The Japanese journal of zootechnical science  

    日本畜産學會報 = The Japanese journal of zootechnical science 70(4), 219-226, 1999-07-25 

    Japanese Society of Animal Science

参考文献:  23件

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被引用文献:  2件

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各種コード

  • NII論文ID(NAID)
    10021723947
  • NII書誌ID(NCID)
    AN00195188
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    1346907X
  • データ提供元
    CJP書誌  CJP引用  J-STAGE 
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