Collagen-Carboxymethyl Dextran Conjugate without Platelet Aggregation
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- TOYOKAWA Tetsuya
- Faculty of Agriculture, Tokyo University of Agriculture and Technology Okinawa Industrial Technology Center
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- HATTORI Makoto
- Faculty of Agriculture, Tokyo University of Agriculture and Technology
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- TAKAHASHI Koji
- Faculty of Agriculture, Tokyo University of Agriculture and Technology
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Abstract
Pepsin solubilized collagen-carboxymethyl dextran (PSC-CMD) conjugate was prepared to improve the function of collagen by using cyanogen bromide. The conjugation between PSC and CMD was confirmed by SDS-PAGE, isoelcctric focusing and size-exclusion chromatography. The molar ratio of the conjugate was PSC:CMD=1:2.3. The isoelectric point was shifted from 9.0 to 5.5 by conjugation. Denaturation temperature of the conjugate molecule was 42.2°C, which is 1.9°C higher than that of PSC. The PSC-CMD conjugate maintained self-assembling ability but showed a very slow aggregation as compared with that of PSC. Reconstructed matrix of the conjugate had lower denaturation temperature and random molecular rearrangement structure as compared with PSC matrix. The platelet aggregation inducing ability, analyzed by spectroscopy disappeared.
Journal
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- Nihon Chikusan Gakkaiho
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Nihon Chikusan Gakkaiho 70 (4), 219-226, 1999
Japanese Society of Animal Science
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Details
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- CRID
- 1390282680173225856
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- NII Article ID
- 10021723947
- 10020393172
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- NII Book ID
- AN00195188
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- ISSN
- 18808255
- 00215309
- 1346907X
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- NDL BIB ID
- 4812845
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed