Preparation and Dynamic Viscoelastic Characterization of Pepsin-Solubilized Collagen from Shark Skin Compared with Pig Skin
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Aiming at optimizing the preparation of pepsin-solubilized collagen and investigating its properties, comparison between shark and pig skin collagens (SC and PC) was done. SC is not only more soluble in acetic acid but also more susceptible to pepsin-attack compared with PC. Solubilization of SC attained almost 100% at 20°C, while PC dissolved about 50%. SC has a small amount of collagen-associated peptides, which appeared transiently released from SC with pepsin at 20°C and disappeared ultimately. They are consisted of non-collagen and collagen origins. For preparation of pepsin-solubilized SC (PSSC), it is desirable to conduct the pepsin treatment at 20°C for more than 48 hours. Pepsin-solubilized PC (PSPC) mainly consisted of α chain, while PSSC mainly consisted of β chain. Elastic modulus G' and viscous modulus G" of collagen solution decreased suddenly with concomitant rapid increase in tan δ at a defined temperature, reflecting the typical denaturation process of collagen. G" of PSSC and PSPC was smaller than that of acid soluble collagen (ASC), and tan δ of PSSC and PSPC was also higher than that of ASC. It is suggested that the decrease of intermolecular interaction by removing telopeptide with pepsin. It is considered that the present dynamic viscoelasticity study could distinguish the difference between PSSC and PSPC, and hence provides an effective means of evaluating collagen materials.
- Nihon Chikusan Gakkaiho
Nihon Chikusan Gakkaiho 70(4), 227-234, 1999-07-25
Japanese Society of Animal Science