Effects of Seminal Plasma Components on Motility and Acrosomal Integrity of Meishan Boar Spermatozoa after Cooling Treatments

• HARAYAMA Hiroshi

  Graduate School of Science and Technology, Kobe University

• IMANO Akira

  Faculty of Agriculture, Kobe University

• MIYAKE Masashi

  Graduate School of Science and Technology, Kobe University

• KATO Seishiro

  Faculty of Agriculture, Kobe University

Bibliographic Information

Other Title

• 冷却処理後の梅山豚射出精子の運動性および先体の形態に及ぼす精漿成分の影響
• Effects of Seminal Plasma Components on

Abstract

This study was conducted to determine effects of seminal plasma components on maintaining motility and acrosomal integrity in Meishan boar spermatozoa during cooling treatments. Ejaculates were collected from a Meishan boar by a manual method. Seminal plasma components were separated by dialysis and anion-exchange column chromatography. Spermatozoa resuspended in TCG solution containing seminal plasma or its components were cooled slowly to 4°C, or rapidly to 0°C, after which sperm motility and acrosomal integrity were examined. For those samples that were cooled slowly, the addition of seminal plasma (5-20%, v/v) exerted protective effects on maintaining sperm motility, but most spermatozoa were immobile at a high concentration (80%). However, the deleterious effects on sperm motility were not detected in seminal plasma dialyzed against TCG solution. When seminal plasma components were separated into four fractions (Fractions 1-4) by anion-exchange column chromatography, the protective effects were detected in Fractions 2 and 4. However, seminal plasma and its components hardly affected acrosomal integrity in the spermatozoa that were cooled slowly. For sperm samples that were cooled rapidly, the protective effects on acrosomal integrity as well as on sperm motility were observed in Fractions 2 and 4. These results imply that Meishan boar seminal plasma contains both beneficial and detrimental components for maintaining sperm motility during the cooling treatments. The formers are characterized by the affinity to the anion-exchange column, i.e., molecules with isoelectric points in acidic and/or neutral ranges, and the latters are passable through the dialysis tubing, i.e., low-molecular-mass molecules.

Journal

• Nihon Chikusan Gakkaiho

  Nihon Chikusan Gakkaiho 69 (8), 720-727, 1998

  Japanese Society of Animal Science

Keywords

• Seminal plasma
• Cooling resistance
• Spermatozoa
• Boar

Details

• CRID: 1390001205196095616
• NII Article ID: 130000743089
• NII Article ID: 10021725296
• NII Article ID: 10020394521
• NII Book ID: AN00195188
• DOI: 10.2508/chikusan.69.720
• ISSN: 18808255
• ISSN: 00215309
• ISSN: 1346907X
• NDL BIB ID: 4546359
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4546359
• Web Site: https://search.jamas.or.jp/link/ui/1999056128
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed