ラット初期胚の体外発育に及ぼすグルコースおよびアミノ酸の影響

DOI Web Site 被引用文献1件 参考文献33件

書誌事項

タイトル別名
  • Effects of Glucose and Amino Acids on In-Vitro Development of Rat Preimplantation Embryos, and Morphological Features of the Developed Blastocysts
  • Effects of Glucose and Amino Acids on I

この論文をさがす

抄録

This study examined the effects of glucose and amino acids on in vitro development of Sprague-Dawley (SD) rat 1-cell embryos, and the morphological features of the developed blastocysts. Approximately 60% of one-cell embryos developed in vitro to the blastocyst stage when 0.5mM glucose was added to a chemically defined medium (modified hamster embryo culture medium-1; HECM-1), and glucose and amino acids had synergistic effects on the development. However, the mean cell number of the blastocysts developed in vitro from 1-cell stage embryos was significantly less compared with that of the blastocysts developed in vivo (P<0.01). The morphological features of 1-cell embryos developed in vitro to the blastocyst stage differed somewhat to those of the in vivo-developed blastocysts; less development of blastocoele, narrow intercellular space in inner cell mass and high electron-dense mitochondria. These results suggest that SD rat 1-cell embryos can developed in vitro to the blastocysts stage, and glucose and amino acids have important roles in the embryonic development. However, the blastocysts developed in vitro from 1-cell embryos may have some differences in comparison with the blastocysts developed in vivo.

収録刊行物

  • 日本畜産学会報

    日本畜産学会報 69 (7), 637-645, 1998

    公益社団法人 日本畜産学会

被引用文献 (1)*注記

もっと見る

参考文献 (33)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ