Prediction of Bovine Blood Plasma Cholesterol by Near Infrared Spectrophotometry

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  • 近赤外スペクトルによるウシ血漿コレステロールの予測
  • Prediction of Bovine Blood Plasma Chole

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Abstract

A near infrared spectrophotometry method is one of the nondestructive measurement techniques of live body information, and development and application of new technique are advanced. If the relevance of the composition and the near infrared spectra of blood plasma of animals is found out, rapid measurement of blood plasma composition will be attained. One hundred and ninety blood samples were collected from beef cattle and dairy cows, and the blood plasma were analyzed. The analyzed blood plasma compositions were as follows; total protein (TP), albumin (Alb), urea nitrogen (UN), glucose (Glu), tri-glyceride (TG), phospho-lipid (PL), free cholesterol (Fcho), total cholesterol (Tcho), inorganic phosphorus (P), calcium (Ca), sodium (Na), potassium (K), and chlorine (Cl). After thawing the samples, visible and the near infrared spectra (400-2500nm) of transmittable light were measured by the near infrared spectrophotometer (NIRSystems 6500) at intervals of the wavelength of 2nm. The first and second derivatives of the spectra were calculated. After, raw spectra and derivatives were made into independent variables, and all blood plasma compositions were set to objective variables, stepwise multiple regression analysis by SAS was performed. Consequently, 0.95 or more coefficients of determination of an estimation formula were obtained for Tcho, Fcho, and PL after 5 steps of the multiple regression analysis. Furthermore, 0.97 or more coefficients of determination were obtained from the result of a validation by taking in seven or more variables of first and second derivative to the prediction equation for Tcho and Fcho. When five or more independent variables were taken in by the prediction equation of PL after a validation, 0.97 or more coefficients of determination were obtained. On the one hand, when the prediction equations for UN, Alb, and Glu took in four or more independent variables, the coefficient of determination after validation exceeded 0.5 slightly. From the above results, it was shown that prediction of Tcho, Fcho and PL in the blood plasma by the near infrared spectra is possible.

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