Ascorbic Acid-2-Phosphate Enhances Adipocyte Differentiation of Cultured Stromal Vascular Cells Prepared from Bovine Perirenal Adipose Tissue

• TORII Shin-ichiro

  Graduate School of Agriculture, Kyoto University

• OHYAMA Michiyo

  Graduate School of Agriculture, Kyoto University

• MATSUI Tohru

  Graduate School of Agriculture, Kyoto University

• YANO Hideo

  Graduate School of Agriculture, Kyoto University

Bibliographic Information

Other Title

• アスコルビン酸-2-リン酸はウシ腎臓脂肪組織から調製した間質血管系細胞群の脂肪細胞への分化を促進する
• Ascorbic Acid 2 Phosphate Enhances Adip

Abstract

Stromal vascular cells isolated from perirenal adipose tissue of adult beef cattle were cultured, and the actions of L-ascorbic acid-2-phosphate (Asc-P) on the adipocyte differentiation of these cells were investigated. The addition of Asc-P into culture medium increased adipogenic marker enzyme (glycerol-3-phosphate dehydrogenase) activity of the cells as well as the number of lipid-laden cells countable under microscopic observation, at concentrations of 50 and 500μM. Cytosolic protein content was unaffected by the Asc-P treatments. In conclusion, Asc -P enhances adipocyte differentiation of bovine preadipocytes in vitro. The results raise the possibility that adipose tissue growth in beef or dairy cattle could be manipulated by controlling blood levels of vitamin C.

Journal

• Nihon Chikusan Gakkaiho

  Nihon Chikusan Gakkaiho 69 (5), 439-444, 1998

  Japanese Society of Animal Science

Keywords

• Preadipocyte
• Adipocyte differentiation
• Vitamin C

Details

• CRID: 1390001205196171136
• NII Article ID: 10021726101
• NII Article ID: 10020395326
• NII Book ID: AN00195188
• DOI: 10.2508/chikusan.69.439
• ISSN: 18808255
• ISSN: 00215309
• ISSN: 1346907X
• NDL BIB ID: 4482261
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4482261
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed