Effects of Population Size and Marker Interval on Efficiency of Constructing a Genetic Linkage Map Using Reference Family from lntercross

• SATOH Masahiro

  National Institute of Animal Industry

• FURUKAWA Tsutomu

  National Institute of Animal Industry

Bibliographic Information

Other Title

• 二重相互交配による標準家系の大きさおよび染色体上のマーカーの間隔が連鎖解析の効率に及ぼす影響

Abstract

Effects of population size, and interval of markers on a chromosome in reference family on the efficiency of linkage analysis was studied using computer simulation. The genetic linkagemap was constructed using a two generation reference population of intercross. Three types of reference populations were used for investigating the effect of reference population size: The first comprised 6 F₁ sires and 10 different numbers of F₁ dams (6 to 60) derived from a cross between three grandsires from a different breed with six grandams from another breed. Each F₁ dam produced eight progenies. The second comprised 12 F₁ sires and 12, 24, 48 or 96 F₁ dams derived from a cross between six grandsires and 12 grandams. F₁ dams produced 192 F2 progenies altogether. The third comprised 24 F₁ dams and 12 or 24 F₁ sires derived from across between 1, 2 or 3 grandsires and 6 or 12 grandams and each F₁ dam produced eight progenies. All F₁ dams were mated randomly to each type of F₁ sires from reference population. Chromosomal map length of 1.2 Morgans, and an average interval between markers of 0.05, 0.10, 0.15 or 0.20 Morgans on a chromosome and two alleles of markers were assumed. The considered markers were placed at random intervals on a chromosome. Chromosomal map length and recombination rate between markers with the lod scores (Z) were estimated using genotypic data from the reference population. Efficiency of linkage analysis increased consistently, but the rate declined steadily as the number of F2 animals increased. The shortest average interval between markers was not always the best efficiency for mean square error of chromosomal length and rate of no double heterozygote between markers. The efficiency of linkage analysis increased slightly as the number of grandparents and F₁ sires increased when number of F₁ dams (F2 animals) was kept constant.

Journal

• Nihon Chikusan Gakkaiho

  Nihon Chikusan Gakkaiho 69 (2), 126-132, 1998

  Japanese Society of Animal Science

Keywords

• Linkage analysis
• Population size
• Interval of markers
• DNA markers

Details

• CRID: 1390282680172331648
• NII Article ID: 10021726831
• NII Book ID: AN00195188
• DOI: 10.2508/chikusan.69.126
• ISSN: 18808255
• ISSN: 1346907X
• Text Lang: ja
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed