A simple filtration device for separating nonadherent microorganisms from mammalian cells in in vitro studies on microbial adherence

• Arseculeratne Sarath N.

  Department of Microbiology, Faculty of Medicine, University of Peradeniya

• Sumathipala Saranga

  Department of Microbiology, Faculty of Medicine, University of Peradeniya

• Eriyagama Navaratne B.

  Department of Microbiology, Faculty of Medicine, University of Peradeniya

抄録

In adherence studies, the removal of nonadherent microorganisms is essential for the valid enumeration of microorganisms that adhere to host cells. Although filtration devices are available commercially for the removal of nonadherent microorganisms, these are expensive and not reusable. In this article, we describe a simple, inexpensive, and reusable filtration device composed of two chambers of nylon, a nylon membrane of desired pore size, a rubber washer, and supporting stainless steel mesh. The device was effective in in vitro adherence assays for removing nonadherent endospores of Rhinosporidium seeberi from human buccal epithelial cells, providing valid counts of adherent microorganisms.

収録刊行物

• Mycoscience

  Mycoscience 49 (5), 339-341, 2008

  一般社団法人 日本菌学会

キーワード

• Adherence assays in vitro
• Filtration device
• <i>Rhinosporidium seeberi</i>

詳細情報

• CRID: 1390577078292231808
• NII論文ID: 10021906478
• NII書誌ID: AA11003436
• DOI: 10.47371/mycosci.myc49339
• ISSN: 16182545
• ISSN: 13403540
• NDL書誌ID: 9661688
• Web Site: http://id.ndl.go.jp/bib/9661688
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I9661688
• Web Site: https://www.jstage.jst.go.jp/article/mycosci/49/5/49_MYC49339/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用可