Rapid enumeration of sulphate-reducing bacteria from aquatic environments using real-time PCR

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We describe a rapid and simple enumeration method for sulphate-reducing bacteria (SRB) from aquatic environments using a quantitative real-time PCR (qPCR) in combination with a rapid DNA isolation method. Enumeration of SRB in the sediment and water samples was performed by quantifying the copy number of the dsrA gene coding for the α-subunit of the dissimilatory sulphite reductase using real-time PCR with the SYBR Green I assay. Using dsrA-specific primers, we demonstrated that quantification of SRB in known numbers of SRB assemblages can be achieved. We compared DNA isolation methods using commercial DNA extraction kits and a published technique. We found two commercial kits were of advantage for extraction of DNA from water or sediment samples, where a large number of samples could be processed at the same time. We showed this newly developed qPCR technique targeting dsrA is rapid, simple and reproducible for the quantification of SRB numbers in situ and is superior to the use of culture-dependent techniques.

収録刊行物

  • Plankton & benthos research  

    Plankton & benthos research 3(3), 180-183, 2008-08-01 

    日本プランクトン学会、日本ベントス学会

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被引用文献:  4件

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各種コード

  • NII論文ID(NAID)
    10021907601
  • NII書誌ID(NCID)
    AA12130745
  • 本文言語コード
    ENG
  • 資料種別
    NOT
  • ISSN
    18808247
  • NDL 記事登録ID
    9638127
  • NDL 雑誌分類
    ZR1(科学技術--生物学)
  • NDL 請求記号
    Z54-J424
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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