Molecular characterization of the flavonoid biosynthetic pathway and flower color modification of Nierembergia sp
-
- Ueyama Yukiko
- Division of Molecular Science, Graduate School of Science and Technology, Kobe University
-
- Katsumoto Yukihisa
- Institute for Advanced Technology, Suntory, Ltd.
-
- Fukui Yuko
- Institute for Advanced Technology, Suntory, Ltd.
-
- Fukuchi-Mizutani Masako
- Institute for Advanced Technology, Suntory, Ltd.
-
- Ohkawa Hideo
- Institute for Advanced Technology, Suntory, Ltd.
-
- Kusumi Takaaki
- Institute for Advanced Technology, Suntory, Ltd.
-
- Iwashita Takashi
- Suntory Institute for Bioorganic Research
-
- Tanaka Yoshikazu
- Institute for Advanced Technology, Suntory, Ltd.
この論文をさがす
抄録
Nierembergia sp., a popular floricultural species, only has violet and white flower color and lacks pink to red. To elucidate the reason, we analyzed its flavonoids. Its major anthocyanin was determined to be delphinidin 3-O-(6-O-(4-O-(4-O-(6-O-caffeoyl-β-D-glucopyranosyl)-p-coumaroyl)-α-L-rhamnosyl)-β-D-glucopyranosyl)-5-O-β-D-glucopyranoside. The petals rarely contained cyanidin and pelargonidin, and they contained more flavonols than anthocyanins. We also characterized the biosynthetic pathway by cloning the cDNAs encoding enzymes involved in the flavonoid biosynthesis pathway: chalcone synthase (CHS), flavanone 3-hydroxylase (F3H), flavonoid 3′-hydroxylase (F3′H), flavonoid 3′,5′-hydroxylase (F3′5′H), dihydroflavonol 4-reductase (DFR), flavonol synthase (FLS), and UDP-rhamnose: anthocyanidin 3-glucoside rhamnosyltransferase (3RT). Northern blot analysis revealed that the expressions of CHS, F3′5′H, DFR, and 3RT genes were coordinately regulated in parallel with anthocyanin accumulation in the petals, indicating that anthocyanin biosynthesis is transcriptionally regulated; on the other hand, the transcripts of the F3′H gene were rarely detected. Antisense suppression of the F3′5′H gene decreased the amount of F3′5′H transcripts and that of delphinidin. It was noteworthy that the color of the transgenic flower changed from violet to white rather than to reddish, which was the expectation.
収録刊行物
-
- Plant Biotechnology
-
Plant Biotechnology 23 (1), 19-24, 2006
日本植物バイオテクノロジー学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390282679303961088
-
- NII論文ID
- 10021907711
-
- NII書誌ID
- AA11250821
-
- COI
- 1:CAS:528:DC%2BD28XktFaqsrk%3D
-
- ISSN
- 13476114
- 13424580
-
- NDL書誌ID
- 7857078
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
-
- 抄録ライセンスフラグ
- 使用不可