Dominant inheritance of white-flowered and herbicide-resistant traits in transgenic gentian plants

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To produce white-flowered gentian plants, we attempted to suppress the chalcone synthase (<i>chs</i>) gene by <i>Agrobacterium</i>-mediated transformation. A binary vector, pSMABcCHS, harboring antisense cDNA of <i>chs</i> isolated from the gentian <i>Gentiana triflora</i> cv. Maciry under control of the CaMV35S promoter was transformed into an interspecific hybrid gentian (cv. Albireo; <i>G. scabra</i>×<i>G. triflora</i>). The vector also contained the <i>bar</i> gene as a selectable marker. Three out of seventeen transgenic plants showed completely white flowers with 10 to 25% reduced anthocyanin content compared with the wild-type. Molecular analyses confirmed integration of the foreign genes and suppression of <i>chs</i> mRNA accumulation in their petals. Application of commercial herbicide including bialaphos showed that the transformants were strongly resistant. Segregation of the white-flowered and herbicide-resistant traits was tested using a T<sub>1</sub> progeny obtained from crossing with a blue-flowered parental line. The results clearly showed that these two traits are inherited dominantly as linked traits in the T<sub>1</sub> progeny, suggesting that these transgenic plants are useful resources for production of white-flowered gentians. These results also demonstrated for the first time the inheritance of foreign genes and genetic modification of flower color in transgenic gentian plants.

収録刊行物

  • Plant biotechnology  

    Plant biotechnology 23(1), 25-31, 2006-03-01 

    Japanese Society for Plant Cell and Molecular Biology

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各種コード

  • NII論文ID(NAID)
    10021907745
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13424580
  • NDL 記事登録ID
    7857086
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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