Dominant inheritance of white-flowered and herbicide-resistant traits in transgenic gentian plants
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To produce white-flowered gentian plants, we attempted to suppress the chalcone synthase (<i>chs</i>) gene by <i>Agrobacterium</i>-mediated transformation. A binary vector, pSMABcCHS, harboring antisense cDNA of <i>chs</i> isolated from the gentian <i>Gentiana triflora</i> cv. Maciry under control of the CaMV35S promoter was transformed into an interspecific hybrid gentian (cv. Albireo; <i>G. scabra</i>×<i>G. triflora</i>). The vector also contained the <i>bar</i> gene as a selectable marker. Three out of seventeen transgenic plants showed completely white flowers with 10 to 25% reduced anthocyanin content compared with the wild-type. Molecular analyses confirmed integration of the foreign genes and suppression of <i>chs</i> mRNA accumulation in their petals. Application of commercial herbicide including bialaphos showed that the transformants were strongly resistant. Segregation of the white-flowered and herbicide-resistant traits was tested using a T<sub>1</sub> progeny obtained from crossing with a blue-flowered parental line. The results clearly showed that these two traits are inherited dominantly as linked traits in the T<sub>1</sub> progeny, suggesting that these transgenic plants are useful resources for production of white-flowered gentians. These results also demonstrated for the first time the inheritance of foreign genes and genetic modification of flower color in transgenic gentian plants.
- Plant tissue culture letters
Plant tissue culture letters 23(1), 25-31, 2006-03-01
Japanese Society for Plant Cell and Molecular Biology