Production of dwarf potted gentian using wild-type Agrobacterium rhizogenes

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We successfully produced dwarf potted gentian using wild-type <i>Agrobacterium rhizogenes</i> A4 strain (ATCC43057) harboring an agropine type plasmid (pRiA4). A number of hairy roots were induced by direct inoculation with <i>A. rhizogenes</i> to the stem and leaf tissues <i>in vitro</i>. Adventitious shoots were regenerated from the hairy roots on Murashige and Skoog medium containing 10 mg l<sup>−1</sup> N-phenyl-N′-(1,2,3-thiadiazol-5-yl) urea, 0.1 or 1.0 mg l<sup>−1</sup> 1-naphthaleneacetic acid, and 3 g l<sup>−1</sup> gellan gum. Regenerated plants were then cultured <i>in vitro</i> for at least six months and tested for the absence of viable <i>A. rhizogenes</i> within their tissues. After receiving authorization from the Ministry of Agriculture, Forestry and Fisheries, Japan, the plants were acclimated and grown in a greenhouse and/or field. Opine synthesis and <i>rolC</i> gene expression were analyzed to demonstrate successful introduction and expression of the transferred DNA. In total, 122 lines of variant types of dwarf plants with blue, white, and pink flowers were obtained, which might be useful for molecular breeding of a series of dwarf potted gentian cultivars.

収録刊行物

  • Plant biotechnology  

    Plant biotechnology 23(1), 33-38, 2006-03-01 

    Japanese Society for Plant Cell and Molecular Biology

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各種コード

  • NII論文ID(NAID)
    10021907791
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13424580
  • NDL 記事登録ID
    7857090
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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