Production of dwarf potted gentian using wild-type Agrobacterium rhizogenes
We successfully produced dwarf potted gentian using wild-type <i>Agrobacterium rhizogenes</i> A4 strain (ATCC43057) harboring an agropine type plasmid (pRiA4). A number of hairy roots were induced by direct inoculation with <i>A. rhizogenes</i> to the stem and leaf tissues <i>in vitro</i>. Adventitious shoots were regenerated from the hairy roots on Murashige and Skoog medium containing 10 mg l<sup>−1</sup> N-phenyl-N′-(1,2,3-thiadiazol-5-yl) urea, 0.1 or 1.0 mg l<sup>−1</sup> 1-naphthaleneacetic acid, and 3 g l<sup>−1</sup> gellan gum. Regenerated plants were then cultured <i>in vitro</i> for at least six months and tested for the absence of viable <i>A. rhizogenes</i> within their tissues. After receiving authorization from the Ministry of Agriculture, Forestry and Fisheries, Japan, the plants were acclimated and grown in a greenhouse and/or field. Opine synthesis and <i>rolC</i> gene expression were analyzed to demonstrate successful introduction and expression of the transferred DNA. In total, 122 lines of variant types of dwarf plants with blue, white, and pink flowers were obtained, which might be useful for molecular breeding of a series of dwarf potted gentian cultivars.
- Plant biotechnology
Plant biotechnology 23(1), 33-38, 2006-03-01
Japanese Society for Plant Cell and Molecular Biology