Expression of an enhancin gene from the Trichoplusia granulosis virus confers resistance to lepidopterous insect pests to rice
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- Mori Mari
- Shiga Prefecture Agricultural Technology Promotion Center
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- Kitamura Harushige
- Shiga Prefecture Agricultural Technology Promotion Center
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- Kondo Atsushi
- Shiga Prefecture Government
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- Dohi Koji
- Ishikawa Prefectural University CREST, Japan Science and Technology Agency
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- Mori Masashi
- Ishikawa Prefectural University CREST, Japan Science and Technology Agency
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- Kaido Masanori
- Graduate School of Agriculture, Kyoto University
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- Mise Kazuyuki
- Graduate School of Agriculture, Kyoto University
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- Shimojyo Eiichi
- Faculty of Textile Science, Kyoto Institute of Technology
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- Hashimoto Yoshifumi
- Faculty of Textile Science, Kyoto Institute of Technology
書誌事項
- タイトル別名
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- Expression of an enhancin gene from the Trichoplusia ni granulosis virus confers resistance to lepidopterous insect pests to rice
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抄録
Transgenic plants that produce anti-insect substances are vital in improving crop yields and in reducing the environmental risks of chemical insecticides. Enhancin is a metalloprotease produced in occlusion bodies of the Trichoplusia ni granulovirus (TnGV). It is a key substance that enhances infection of the nucleopolyhedrovirus in lepidopteran insects. Rice (Oryza sativa L. cv. Nipponbare) protoplasts were cotransformed with pREXΦVEF and pLTHyg, which respectively bear the chimeric enhancin gene and the hygromycin-resistance gene. Hygromycin-resistant regeneration plants were examined by genomic polymerase chain reaction and genomic Southern and northern blotting analyses to confirm the presence and expression of the enhancin gene. Fourteen transgenic plant lines harboring the enhancin gene were obtained, and stable inheritance and expression of the enhancin gene were confirmed in the second, third, and fourth plant generations. Feeding Spodoptera exigua larvae leaves of enhancin-expressing rice plants in the presence of S. exigua nucleopolyhedrovirus occlusion bodies enhanced infection of the virus. Further, the development of Pseudaletia separata, S. exigua, and S. litura, none of which are host insects of TnGV, was inhibited when these larvae were fed enhancin-expressing rice leaves. This indicates that expression of the enhancin gene confers resistance to lepidopteran insect pests in rice.
収録刊行物
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- Plant Biotechnology
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Plant Biotechnology 23 (1), 55-61, 2006
日本植物バイオテクノロジー学会
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詳細情報 詳細情報について
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- CRID
- 1390001204327250560
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- NII論文ID
- 10021907868
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- NII書誌ID
- AA11250821
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- COI
- 1:CAS:528:DC%2BD28XktFaqs7w%3D
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- ISSN
- 13476114
- 13424580
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- NDL書誌ID
- 7857119
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可