Genetic studies of transgenic rice plants overproducing an antibacterial peptide show that a high level of transgene expression did not cause inferior effects on host plants

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Overexpression of an oat thionin gene (<i>Asthi1</i>) confers enhanced disease resistance of rice (a cultivar "Chiyohonami") against seed-transmitted phytopathogenic bacteria. The isolated paddy field test for evaluation of agronomic traits using T4 transgenic rice plants which are homozygous with a high level of Asthi1 protein has shown that transgenic rice possesses, in addition to enhanced anti-bacterial resistance, slight different characteristics compared with the original cultivar such as slight decrease in plant height, grain yield and simultaneity in heading behavior in addition to 2 days earlier in heading date. To address whether this difference was caused by ectopic high level expression of the transgene, T4 plants were back-crossed with wild-type and selfed, and the progenies were analyzed. Almost all characteristics observed in the field were found to be genetically segregated. While only a limited number of plants were used, the present findings indicate that the altered characteristics found in transgenic plants were not attributable to integration of <i>Asthi1</i> transgene or its high expression itself, but instead to possible spontaneous mutations which may have occurred during <i>in vitro</i> culture for <i>Agrobacterium</i>-mediated transformation.

収録刊行物

  • Plant biotechnology  

    Plant biotechnology 23(1), 63-69, 2006-03-01 

    Japanese Society for Plant Cell and Molecular Biology

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各種コード

  • NII論文ID(NAID)
    10021907902
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13424580
  • NDL 記事登録ID
    7857135
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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