Regeneration and genetic engineering of a tropical tree, Azadirachta excelsa

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<i>Azadirachta excelsa</i> belongs to the family Meliaceae and is one of the most important silviculture trees in the tropics. In this study, we established a somatic embryogenesis system and succeeded in construction of transgenic lines expressing genes for <i>Bar</i>, <i>GUS</i> and <i>GFP</i> employing the <i>Agrobacterium</i>-mediated transformation technique. Somatic embryos were obtained after approximately two months of culture on Murashige-Skoog (MS) medium containing 5 μM 6-benzyladenine. Plantlets were then regenerated by transferring somatic embryos to modified 1/2 MS medium without phytohormones. We initially attempted genetic transformation using two different <i>A. tumefaciens</i> strains, and found only the strain LBA4404 to be active in infection to explants. The strain EHA101 was totally inactive, suggesting that a specific interaction between <i>Agrobacterium</i> sp. and host plant might be critical for gene transfer. The possibility is discussed of applying the methodology developed in this work to the practical propagation of tropical trees.

収録刊行物

  • Plant biotechnology

    Plant biotechnology 23(1), 123-127, 2006-03-01

    Japanese Society for Plant Cell and Molecular Biology

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各種コード

  • NII論文ID(NAID)
    10021908113
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    SHO
  • ISSN
    13424580
  • NDL 記事登録ID
    7857262
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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