Transgenic potatoes expressing wasabi defensin peptide confer partial resistance to gray mold (Botrytis cinerea)

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Five potato (<i>Solanum tuberosum</i> L.) cultivars were transformed with <i>Agrobacterium tumefaciens</i> strain EHA101 harboring wasabi defensin gene (isolated from <i>Wasabia japonica</i> L.) in a binary plasmid vector, pEKH1. The infected tuber explants co-cultivated for 3 days resulted in higher transformation efficiency (7–50% higher) for all cultivars than 2 days co-cultivation. PCR analysis showed an amplified fragment of wasabi defensin gene and the selectable marker, <i>nptII</i> gene in the genomic DNA of all clones rooted on MS medium supplemented with 100 mgl<sup>−1</sup> kanamycin, suggesting their transgenic nature. Southern blot analysis confirmed that transgenic plants integrated 1–6 copies of wasabi defensin gene into their genome. Expression of wasabi defensin protein was confirmed in the leaf extracts of independent transgenic clones by Western blot analysis. Antifungal assay of detached leaves from non-transformed control and transgenic plants indicated that transgenic plants were partially resistant to the fungal pathogen, <i>Botrytis cinerea</i> (gray mold).

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  • Plant biotechnology  

    Plant biotechnology 23(2), 179-183, 2006-03-01 

    Japanese Society for Plant Cell and Molecular Biology

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各種コード

  • NII論文ID(NAID)
    10021908395
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13424580
  • NDL 記事登録ID
    7857202
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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