Transgenic Phalaenopsis plants with resistance to Erwinia carotovora produced by introducing wasabi defensin gene using Agrobacterium method
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Transgenic plants over-expressing wasabi defensin gene were successfully produced in <i>Phalaenopsis</i> orchid by <i>Agrobacterium</i>-mediated transformation method. Embryogenic cell suspension culture of <i>Phalaenopsis</i> Wataboushi '#6.13' was infected with <i>A. tumefaciens</i> strain EHA101 carrying a plasmid containing wasabi defensin gene and selectable marker <i>npt</i>II, <i>hpt</i> genes. Plantlets were regenerated through somatic embryogenesis from the calli selected on hygromycin-containing medium. Transformation of plantlets with wasabi defensin gene was confirmed by PCR analysis. Southern blot analysis confirmed successful integration of 1–4 copies of the gene. Production of the 5 kDa wasabi defensin protein with varying levels was confirmed in the leaf extracts of different transgenic clones using Western blot analysis. Most of the transgenic plants showed strong resistance to <i>Erwinia carotovora</i>, which causes soft rot disease in the control plant. These results suggest the usefulness of this gene for conferring the resistance to various diseases of <i>Phalaenopsis</i> and possibly other orchids.
- Plant tissue culture letters
Plant tissue culture letters 23(2), 191-194, 2006-03-01
Japanese Society for Plant Cell and Molecular Biology