Non-biased distribution of tomato genes with no counterparts in Arabidopsis thaliana in expression patterns during fruit maturation
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We prepared DNA array nylon filters using 10,905 cDNA clones selected from fruit and leaf cDNA libraries of the miniature tomato (<i>Solanum lycopersicum</i>) cultivar, Micro-Tom, as being representative of non-redundant sequences of 37,972 Micro-Tom expressed sequence tags (ESTs). Tomato gene expression during fruit maturation was analyzed using the array filters. Graphs of gene expression patterns were arranged into a 4×4 array using a self-organizing map algorithm. Using non-redundant sequences generated from 188,024 tomato ESTs, we assigned the cDNA clones on the array filters to 1151 genes that had no counterparts in the <i>Arabidopsis thaliana</i> genome using stringent conditions for e-values of BLAST searching. We found that the expression patterns of these non-<i>Arabidopsis</i> genes were evenly distributed in the self-organizing map, with no statistically significant difference with the distributions of whole genes. These findings suggested that the non-<i>Arabidopsis</i> genes participate in a wide variety of diverse functions during fruit maturation.
- Plant tissue culture letters
Plant tissue culture letters 23(2), 199-202, 2006-03-01
Japanese Society for Plant Cell and Molecular Biology