A promoter analysis of the DcMYB1 gene that encodes the transcriptional activator of the stress-inducible DcPAL1 gene
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DcMYB1, a carrot MYB protein, acts as a transcriptional activator of the stress-inducible carrot <i>PAL</i> gene <i>DcPAL1</i>, and is induced by several environmental cues such as UV-B irradiation and elicitor treatment. Here, the <i>DcMYB1</i> promoter region was isolated, and its putative <i>cis</i>-elements were characterized using several deletion mutants of the <i>DcMYB1</i> gene promoter. This was undertaken with transient expression experiments using carrot protoplasts. The results indicated that GCC-box and one MYC binding sequence might participate in the up-regulation of <i>DcMYB1</i> promoter activity induced by UV-B irradiation, and that the AG-motif-like and/or Box-P might undertake this by elicitor treatment. MYB and/or another MYC binding site neighboring the TATA-box might take part in the negative regulation of promoter activity, and the W-box near the TATA-box might be necessary for the up-regulation of promoter activity by both UV-B irradiation and elicitor treatment.
- Plant tissue culture letters
Plant tissue culture letters 23(2), 203-209, 2006-03-01
Japanese Society for Plant Cell and Molecular Biology