Isolation and expression analysis of phosphate transporter genes from Eucalyptus camaldulensis
Five distinct genomic DNA fragments (<i>EcPT1</i>, <i>EcPT2</i>, <i>EcPT3</i>, <i>EcPT4</i> and <i>EcPT5</i>) encoding phosphate transporters were isolated from <i>Eucalyptus camaldulensis</i>. <i>EcPT2</i> and <i>EcPT3</i> were exclusively expressed in the root, but were not enhanced by phosphate deprivation. The transcript level of <i>EcPT2</i> was much higher than that of <i>EcPT3</i>. The <i>EcPT2</i> is present as a single copy gene in <i>Eucalyptus</i> genome, and phylogenetic analysis and deduced amino acid sequence revealed that <i>EcPT2</i> can be classified into the Pht1 family, a high-affinity phosphate transporter. These results suggest that <i>EcPT2</i> functions in phosphate transport in root tissues not only under low-phosphate conditions as with typical Pht1 family members, but also under high-phosphate conditions.
- Plant biotechnology
Plant biotechnology 23(2), 215-218, 2006-03-01
Japanese Society for Plant Cell and Molecular Biology