Antisense suppression of glutamate decarboxylase in tomato (Lycopersicon esculentum L.) results in accumulation of glutamate in transgenic tomato fruits

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Tomato plants were transformed with a plasmid that contained a the gene for glutamate decarboxylase (GAD) from <i>Lycopersicon esculentum</i> L. coupled, in the antisense orientation, with the constitutively active 35S promoter from cauliflower mosaic virus. Four independent transformants were obtained. In the fruits of these transgenic plants, the level of expression of GAD mRNA was lower than that in non-transgenic plants. When tomatoes were harvested six weeks after the first flowering, we found that the levels of total free amino acids in transgenic fruits were 1.2 to 3.2 times higher than those in non-transgenic plants. In particular, the level of glutamate in fruits of transgenic plants was about twice that in fruits of non-transgenic plants.

収録刊行物

  • Plant biotechnology  

    Plant biotechnology 23(3), 267-274, 2006-06-01 

    Japanese Society for Plant Cell and Molecular Biology

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各種コード

  • NII論文ID(NAID)
    10021908700
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13424580
  • NDL 記事登録ID
    7943830
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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