Antisense suppression of glutamate decarboxylase in tomato (Lycopersicon esculentum L.) results in accumulation of glutamate in transgenic tomato fruits

• Kisaka Hiroaki

  Research Institute for Health Fundamentals, Ajinomoto Co.

• Hiroaki Takao

  Basic Research Laboratories, Ajinomoto Co., Inc.

• Miwa Tetsuya

  Research Institute for Health Fundamentals, Ajinomoto Co.

抄録

Tomato plants were transformed with a plasmid that contained a the gene for glutamate decarboxylase (GAD) from Lycopersicon esculentum L. coupled, in the antisense orientation, with the constitutively active 35S promoter from cauliflower mosaic virus. Four independent transformants were obtained. In the fruits of these transgenic plants, the level of expression of GAD mRNA was lower than that in non-transgenic plants. When tomatoes were harvested six weeks after the first flowering, we found that the levels of total free amino acids in transgenic fruits were 1.2 to 3.2 times higher than those in non-transgenic plants. In particular, the level of glutamate in fruits of transgenic plants was about twice that in fruits of non-transgenic plants.

収録刊行物

• Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ

  Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ 23 (3), 267-274, 2006

  日本植物バイオテクノロジー学会

キーワード

• antisense regulation
• glutamate decarboxylase
• <i>Lycopersicon esculentum</i>
• ripening fruits

詳細情報

• CRID: 1390282679303863040
• NII論文ID: 10021908700
• NII書誌ID: AA11250821
• DOI: 10.5511/plantbiotechnology.23.267
• COI: 1:CAS:528:DC%2BD28XntVWhtLw%3D
• ISSN: 13476114
• ISSN: 13424580
• NDL書誌ID: 7943830
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7943830
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可