Root specific expression of Na^+/H^+ antiporter gene from Synechocystis sp. PCC 6803 confers salt tolerance of tobacco plant

この論文にアクセスする

この論文をさがす

著者

抄録

Tobacco plants were transformed with a Nhap type Na<sup>+</sup>/H<sup>+</sup> antiporter gene, <i>SynnhaP1</i> (slr1595), from a cyanobacterium <i>Synechocystis</i> sp. PCC 6803. Two kinds of promoters, <i>Arabidopsis</i> alcohol dehydrogenase gene promoter (Adh promoter) and CaMV 35S promoter (35S promoter), were used. The transgenic plants driven by Adh promoter accumulated SynNhaP1 proteins only in root whereas the transgenic plants driven by 35S promoter accumulated SynNhaP1 proteins in all tissues. Confocal imaging of SynNhaP1-GFP fusion protein suggests the intracellular localization of SynNhaP1 in plasma membrane. Transgenic plants exhibited higher germination yields, increased biomass during developmental stage, increased seed production, and decreased intracellular Na<sup>+</sup> content under salt-stress conditions. The transgenic plants driven by Adh promoter exhibited similar or slightly higher salt tolerance than that by 35S promoter. These results indicate the importance of expression of Na<sup>+</sup>/H<sup>+</sup> antiporter in root for salt tolerance in plant.

収録刊行物

  • Plant biotechnology  

    Plant biotechnology 23(3), 275-281, 2006-06-01 

    Japanese Society for Plant Cell and Molecular Biology

参考文献:  19件

参考文献を見るにはログインが必要です。ユーザIDをお持ちでない方は新規登録してください。

各種コード

  • NII論文ID(NAID)
    10021908733
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13424580
  • NDL 記事登録ID
    7943858
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  NDL  J-STAGE 
ページトップへ