Root specific expression of Na^+/H^+ antiporter gene from Synechocystis sp. PCC 6803 confers salt tolerance of tobacco plant
Tobacco plants were transformed with a Nhap type Na<sup>+</sup>/H<sup>+</sup> antiporter gene, <i>SynnhaP1</i> (slr1595), from a cyanobacterium <i>Synechocystis</i> sp. PCC 6803. Two kinds of promoters, <i>Arabidopsis</i> alcohol dehydrogenase gene promoter (Adh promoter) and CaMV 35S promoter (35S promoter), were used. The transgenic plants driven by Adh promoter accumulated SynNhaP1 proteins only in root whereas the transgenic plants driven by 35S promoter accumulated SynNhaP1 proteins in all tissues. Confocal imaging of SynNhaP1-GFP fusion protein suggests the intracellular localization of SynNhaP1 in plasma membrane. Transgenic plants exhibited higher germination yields, increased biomass during developmental stage, increased seed production, and decreased intracellular Na<sup>+</sup> content under salt-stress conditions. The transgenic plants driven by Adh promoter exhibited similar or slightly higher salt tolerance than that by 35S promoter. These results indicate the importance of expression of Na<sup>+</sup>/H<sup>+</sup> antiporter in root for salt tolerance in plant.
- Plant biotechnology
Plant biotechnology 23(3), 275-281, 2006-06-01
Japanese Society for Plant Cell and Molecular Biology