Analysis of functional domains and binding sequences of Arabidopsis transcription factor ESR1
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The <i>Arabidopsis ESR1</i> is thought to be a key gene for commitment to <i>in vitro</i> shoot regeneration in tissue culture. The <i>ESR1</i> gene encodes a putative transcription factor. We examined the DNA-binding potential for specific sequences. Recombinant ESR1 protein specifically bound to the GCC box, known as the ethylene-responsive element. In addition, ESR1 was localized to the nucleus when expressed in onion epidermal cells. Deletion experiments revealed that the DNA-binding domain or the C-terminal region of ESR1 is essential for ESR1 overexpression to enhance shoot regeneration. The C-terminal region may be involved in transcriptional regulation by ESR1. These results suggest that ESR1 functions as a transcription factor and that its transcriptional regulation is important for the initiation of shoot regeneration.
- Plant tissue culture letters
Plant tissue culture letters 23(3), 303-308, 2006-06-01
Japanese Society for Plant Cell and Molecular Biology