A model system to screen for candidate plant activators using an immune-induction system in Arabidopsis
To develop a screening system for plant activators, which are novel substances that protect plants by enhancing their inherent disease-resistance mechanisms, we utilized a <i>GUS</i> reporter gene system using promoters of the defense-related genes, <i>PR-1</i> and <i>PR-4</i>. To validate the strategy, we performed subsequent analysis using an <i>Arabidopsis</i> microarray consisting of 1200 full-length cDNA clones representing putative defense-related and regulatory genes. Benzo(1,2,3)thiadiazole-7-carbothioic acid <i>S</i>-methyl ester and 2,6-dichloroisonicotinic acid activated plant defense responses via the salicylic acid (SA)-dependent signaling pathway, and β-aminobutyric acid triggered a primed state in the plant that enables more efficient activation of the SA-, jasmonic acid- and ethylene-signaling pathway. These results suggest that this novel system can be used to screen for candidate plant activators.
- Plant biotechnology
Plant biotechnology 23(3), 321-327, 2006-06-01
Japanese Society for Plant Cell and Molecular Biology