Cryopreservation of tobacco BY-2 suspension cell cultures by vitrification with encapsulation
Tobacco BY-2 suspension cells were successfully cryopreserved by a vitrification method combined with an encapsulation technique. Cell cultures cryopreserved using the optimal conditions established in this study could be thawed and grown enough to subculture in fresh medium within 14 days. However, the vitrification method was less effective for cryopreservation of BY-2 than a simplified slow prefreezing method.
- Plant biotechnology
Plant biotechnology 23(3), 333-337, 2006-06-01
Japanese Society for Plant Cell and Molecular Biology