Cryopreservation of tobacco BY-2 suspension cell cultures by vitrification with encapsulation

• Kobayashi Toshihiro

  Experimental Plant Division, BioResource Center, RIKEN Tsukuba Institute

• Niino Takao

  Genebank, National Institute of Agrobiological Sciences

• Kobayashi Masatomo

  Experimental Plant Division, BioResource Center, RIKEN Tsukuba Institute

Abstract

Tobacco BY-2 suspension cells were successfully cryopreserved by a vitrification method combined with an encapsulation technique. Cell cultures cryopreserved using the optimal conditions established in this study could be thawed and grown enough to subculture in fresh medium within 14 days. However, the vitrification method was less effective for cryopreservation of BY-2 than a simplified slow prefreezing method.

Journal

• Plant Biotechnology

  Plant Biotechnology 23 (3), 333-337, 2006

  Japanese Society for Plant Biotechnology

Keywords

• cryopreservation
• encapsulation
• slow prefreezing method
• tobacco BY-2
• vitrification method

Details

• CRID: 1390001204327169152
• NII Article ID: 10021908949
• NII Book ID: AA11250821
• DOI: 10.5511/plantbiotechnology.23.333
• COI: 1:CAS:528:DC%2BD28XntVWhur8%3D
• ISSN: 13476114
• ISSN: 13424580
• NDL BIB ID: 7943977
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7943977
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed