Cryopreservation of tobacco BY-2 suspension cell cultures by vitrification with encapsulation

• Kobayashi Toshihiro

  Experimental Plant Division, BioResource Center, RIKEN Tsukuba Institute

• Niino Takao

  Genebank, National Institute of Agrobiological Sciences

• Kobayashi Masatomo

  Experimental Plant Division, BioResource Center, RIKEN Tsukuba Institute

抄録

Tobacco BY-2 suspension cells were successfully cryopreserved by a vitrification method combined with an encapsulation technique. Cell cultures cryopreserved using the optimal conditions established in this study could be thawed and grown enough to subculture in fresh medium within 14 days. However, the vitrification method was less effective for cryopreservation of BY-2 than a simplified slow prefreezing method.

収録刊行物

• Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ

  Ｐｌａｎｔ　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ 23 (3), 333-337, 2006

  日本植物バイオテクノロジー学会

キーワード

• cryopreservation
• encapsulation
• slow prefreezing method
• tobacco BY-2
• vitrification method

詳細情報

• CRID: 1390001204327169152
• NII論文ID: 10021908949
• NII書誌ID: AA11250821
• DOI: 10.5511/plantbiotechnology.23.333
• COI: 1:CAS:528:DC%2BD28XntVWhur8%3D
• ISSN: 13476114
• ISSN: 13424580
• NDL書誌ID: 7943977
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7943977
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可