Biochemical characterization and mutational studies of a chalcone synthase from yellow snapdragon (Antirrhinum majus) flowers

この論文にアクセスする

この論文をさがす

著者

抄録

The 4′-<i>O</i>-glucosides of 2′,4,4′,6′-tetrahydroxychalcone (THC) and 2′,3,4,4′,6′-pentahydroxychalcone (PHC) are present in the yellow snapdragon (<i>Antirrhinum majus</i>) flowers and serve as direct precursors for the synthesis of aurones, the yellow pigments of the flowers. Despite the metabolic significance of these chalcones in aurone biosynthesis, the biochemical properties of chalcone synthase (CHS), the enzyme responsible for their synthesis, remains to be clarified. In this study, one known CHS cDNA (<i>niv</i>, also termed <i>AmCHS1</i>) and one CHS-related cDNA, <i>AmCHS2</i>, were isolated from yellow snapdragon buds. <i>AmCHS1</i> mRNA specifically accumulated in the petals in a coloration-dependent manner, whereas <i>AmCHS2</i> mRNA accumulated only negligibly in petals and other organs, corroborating the importance of AmCHS1 in chalcone synthesis in the yellow flower. Recombinant AmCHS1 that was heterologously expressed in <i>Escherichia coli </i>cells efficiently utilized both <i>p</i>-coumaroyl-CoA and caffeoyl-CoA (relative activity, 50% of the activity for <i>p</i>-coumaroyl-CoA) as a starting ester to produce THC and PHC, respectively. Previous studies predicted that a single Thr197Ala or the double Val196Met/Thr197Ala substitution in AmCHS1 may provide a wider space for hydroxycinnamoyl-group binding and might enhance the ability of this enzyme to produce PHC [Austin MB, Noel JP (2003) <i>Nat Prod Rep</i> 20: 79–110]. However, these substitutions led to a decreased PHC-producing activity, implying that other factors may also be important for the efficient utilization of caffeoyl-CoA.

収録刊行物

  • Plant biotechnology  

    Plant biotechnology 23(4), 373-378, 2006-09-01 

    Japanese Society for Plant Cell and Molecular Biology

参考文献:  22件

参考文献を見るにはログインが必要です。ユーザIDをお持ちでない方は新規登録してください。

被引用文献:  2件

被引用文献を見るにはログインが必要です。ユーザIDをお持ちでない方は新規登録してください。

各種コード

  • NII論文ID(NAID)
    10021909064
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13424580
  • NDL 記事登録ID
    8041088
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
ページトップへ