Increase in peroxidase activity in tea callus in response to darkness, 2,4-dichlorophenoxyacetic acid and carbohydrates

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The peroxidase (POD) reaction for the industrial synthesis of etoposide proceeds effectively when suspension-cultured plant cells are used as catalysts. Tea (<i>Camellia sinensis</i>) suspension cultures express higher POD activity and produce a higher yield of reaction products than those of other plant cells. We intend to investigate the conditions necessary to efficiently increase the POD activity in a suspension-cultured tea callus. Here we examined the influence of photoenvironmental conditions, phytohormones, and carbohydrates on POD activity in the callus grown on solidified medium. POD activity in the callus was increased 1) in the dark; 2) on medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) at 10 mg l<sup>−1</sup>; or 3) on medium containing 0.3 M sucrose and 0.1 M mannitol. The highest POD activity, achieved under the third set of conditions, was about 3.6-fold higher than that achieved on control medium (with 0.09 M sucrose).

収録刊行物

  • Plant biotechnology  

    Plant biotechnology 23(4), 405-408, 2006-09-01 

    Japanese Society for Plant Cell and Molecular Biology

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各種コード

  • NII論文ID(NAID)
    10021909206
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    SHO
  • ISSN
    13424580
  • NDL 記事登録ID
    8041143
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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