An efficient transient gene expression system using aleurones of diploid wheat seeds
To establish a transient gene expression system for investigating the molecular function of genes in mature wheat seeds, we bombarded embryos or aleurones of three hexaploid wheat (<i>Triticum aestivum</i> L.) varieties Chinese Spring, RL4137 and OS21-5, two diploid wheat (<i>T. monococcum</i> L.) varieties KT3-1 and KT3-5 and a barley (<i>Hordeum vulgare</i>, L.) variety Himalaya with fusion constructs containing the GUS reporter gene driven by the <i>Actin1</i> promoter or α-<i>amylase</i> promoter using a particle gun, and compared the efficiency of gene expression for the transient assay. The efficiency of transient expression was high in aleurone tissues of OS21-5, KT3-1, KT3-5 and Himalaya. In the aleurone tissues of KT3-5, the α-<i>amylase</i> promoter was especially markedly activated by gibberellic acid (GA) and by a GA-inducible MYB transcription activator of <i>T. monococcum</i>, TmGAMYB. This assay system using the aleurone tissues of KT3-5 may be especially useful for investigating which genes are expressed and how they are regulated in germinating wheat seeds.
- Plant biotechnology
Plant biotechnology 23(4), 413-417, 2006-09-01
Japanese Society for Plant Cell and Molecular Biology