Cloning and molecular analysis of radish (Raphanus sativus L.) cDNAs encoding heterodimeric γ-glutamyltransferases

この論文にアクセスする

この論文をさがす

著者

抄録

γ-Glutamyltransferase (GGT) catalyzes the hydrolysis and transpeptidation of the γ-glutamyl moiety of γ-glutamyl peptides. Based on the N-terminal amino acid sequences of purified radish heterodimeric GGTs, we cloned and characterized three radish full-length cDNAs (<i>RsGGT1</i>, <i>RsGGT2</i> and <i>RsGGT3</i>) encoding putative heterodimeric GGT isoforms. RsGGT proteins contained conserved amino acid residues that are required for the catalytic activity and the post-translational processing of GGT proteins in <i>E. coli</i> and mammals. Expression analysis indicated that <i>RsGGT</i> showed different organ expression patterns. The overexpression of <i>RsGGT1</i> and <i>RsGGT2</i> cDNAs, but not that of <i>RsGGT3</i> cDNA, resulted in an increase of NaCl-extractable bound GGT activity in transgenic tobacco plants. These results suggest that <i>RsGGT1</i> and <i>RsGGT2</i> cDNAs encode heterodimeric bound GGT isoforms.

収録刊行物

  • Plant biotechnology  

    Plant biotechnology 23(4), 419-424, 2006-09-01 

    Japanese Society for Plant Cell and Molecular Biology

参考文献:  23件

参考文献を見るにはログインが必要です。ユーザIDをお持ちでない方は新規登録してください。

各種コード

  • NII論文ID(NAID)
    10021909262
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    NOT
  • ISSN
    13424580
  • NDL 記事登録ID
    8041332
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  NDL  J-STAGE 
ページトップへ