The combined effect of photoperiod, light intensity and GA_3 on adventitious shoot regeneration from cotyledons of spinach (Spinacia oleracea L.)
The effects of photoperiod, light intensity and gibberellic acid (GA<sub>3</sub>) on adventitious shoot regeneration from spinach cotyledons were tested. The Analysis of Variance F test showed that the effects of photoperiod and GA<sub>3</sub> on shoot regeneration were significant at high light intensity of 90–100 μmol m<sup>−2 s</sup>−1. The combined effect of optimum shoot regeneration and highest shoot multiplication was observed in explants of short day (SD)-grown seedlings cultured under the SD condition and at high light intensity, with 0.5 mg l<sup>−1</sup> GA<sub>3</sub> in Murashige and Skoog medium supplemented with 1 mg l<sup>−1</sup> 6-benzyladenine and 0.4 mg l<sup>−1</sup> α-naphthaleneacetic acid. Scanning electron microscopy observation revealed multiple shoots regenerating directly from the basal end of cotyledon preceding callus. The regenerated shoots developed into normal plantlets and grew well upon transfer to pots. Some regenerated shoots flowered <i>in vitro</i> under the non-inductive SD condition.
- Plant biotechnology
Plant biotechnology 23(4), 431-435, 2006-09-01
Japanese Society for Plant Cell and Molecular Biology