Tobacco RETINOBLASTOMA-RELATED protein is phosphorylated by different types of cyclin-dependent kinases during the cell cycle

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Plants contain a regulatory pathway similar to that involving retinoblastoma (RB) protein in animals. Here, we analyze an <i>RB</i> homolog, tobacco <i>RETINOBLASTOMA-RELATED1</i> (<i>NtRBR1</i>), that encodes a protein possessing 13 potential phosphorylation sites for cyclin-dependent kinases (CDKs). We found that 11 synthetic peptides containing sequences from NtRBR1 were phosphorylated differentially by imunoprecipitates of cyclin/CDK complexes extracted from tobacco BY-2 cells. We raised antibodies that specifically recognize these sites of phosphorylation in NtRBR1. Distinct sites were phosphorylated during the cell cycle, suggesting that NtRBR1 is phosphorylated by different types of cyclin/CDK complexes.

収録刊行物

  • Plant biotechnology  

    Plant biotechnology 23(5), 467-473, 2006-12-01 

    Japanese Society for Plant Cell and Molecular Biology

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各種コード

  • NII論文ID(NAID)
    10021909454
  • NII書誌ID(NCID)
    AA11250821
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13424580
  • NDL 記事登録ID
    8571150
  • NDL 雑誌分類
    ZR3(科学技術--生物学--植物)
  • NDL 請求記号
    Z54-J126
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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